Eukaryotic expression of B7.1 extracellular domain and its biological activities
- VernacularTitle:B7.1胞外编码区的真核表达及其生物活性
- Author:
Zhangmin YANG
;
Yili WANG
;
Lsheng SI
- Publication Type:Journal Article
- Keywords:
B7.1;
extracellular domain;
eukaryotic expression;
biological activity
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To eukaryotically exp ress B7.1 extracellular domian and investigate its biological activities. Methods Recombinant expression vector pDisplay/B7.1(V+C) wa s transfected into Hela cells by electroporation method. The expression of B7.1( V+C) was identified by immunohistochemistry and in situ hybridization, respectiv ely. In vitro T lymphocyte activation activity was detected by 3H-TdR incoporation method and the aroused cytotoxicity after mixed culture of pDispla y/B7.1(V+C) transfected Hela cell-T cell was observed by MTT method. R esults B7.1(V+C) was expressed successfully on the surface of Hela c ells. The expressed B7.1(V+C) was able to activate T lymphocytes in the pres ence of anti-CD3 monoclonal antibody. Cytotoxicity could be observed when pDisp lay/B7.1(V+C) transfected Hela cells were mixed and co-cultured with T lymphocy tes. Conclusion Eukaryotically expressed B7.1(V+C) main tains its T lymphocyte costimulatory activity, and B7.1(V+C) can be adopted to construct bifunctional molecules for tumor immunotherapy.
