Signal transduction in TGF-?1-induced airway smooth muscle cell proliferation in rats
- VernacularTitle:TGF-?1诱导的大鼠ASM细胞增殖的信号转导途径
- Author:
Aru LIU
;
Yingge LIU
;
Haowen QI
- Publication Type:Journal Article
- Keywords:
transforming growth factor;
smooth muscle/cytolog y;
extracellular signal-regulated kinase (ERKs)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate possible intracellular s ignal molecules involved in TGF-?-induced airway smooth muscle cell proli feration in rats. Methods The cultured airway smooth muscle cells were divide d into 3 groups: control group (20 mL?L -1FCS/DMEM), 10 ?g?L -1 TGF-?1 group and 10?g?L -1 TGF-?1 /U-0126 (1 ? mol?L -1) group. The proliferation of ASMCs was detected by MTT. Exp ression of phospho-p42/p44 extracellular signal-regulated kinase (ERKs) with i mmunocytochemistry were examined in different groups. A values were detected by image analysis. Results By MTT, A values of 10?g?L -1 TGF- ?1 group (0.36?0.043) were significantly higher than those of control grou p (0.126?0.052, t=5.44,7.62, P
