Study on gene expression profile of subclonal cell lines in two different anchorage-independent uterine cervical carcinoma
- VernacularTitle:不同表型的人宫颈癌细胞亚克隆株的基因表达谱
- Author:
Wei CHEN
;
Xu LI
;
Yucong YANG
;
Xiaoli CHENG
- Publication Type:Journal Article
- Keywords:
cervical carcinoma;
subclone;
cDNA microarray
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the differently expressed in vasion-related genes in two anchorage-independent uterine cervical carcinoma c ell lines derived from the same patient using a cDNA array. Methods Two human uterine cervical carcinoma subclonal cell lines CS03 and CS07 derived from a single donor line CS1213 were established by limited diluting procedure and their cloning efficiency was counted in soft aga r. The expression of cellular adhesive molecules CD44, E-cadherin and Fibroneti n were studied by flow cytometry and immunohistochemical staining. The two cDNA samples retro-transcribed from total RNA derived from CS03 and CS07 cells were screened by a cDNA microarray carrying 1011 human signal transduction and membra ne receptor-associated genes and scanned with a ScanArray 3000 laser scanner. Results The cloning efficiency was 38.5% in CS07 cells and 0 in CS03 cells, respectively. CD44 were 35.1% and 13.7% in CS03 and CS07,respe ctively. The cDNA microarray analysis showed that 12 genes in CS03 were upregula ted compared with CS07, and 21 genes in CS07 were upregulated. The function of a number of differently expressed genes is consistently associated with cell prol iferation, migration, apoptosis, signal transduction and tumor metastasis, inclu ding p34 cdc2, TSC22, plasminogen activator inhibitor I (PAI-1)and desmoso me associated protein (Pinin). Conclusion Multiple genes are differently expressed in uter ine cervical carcinoma cell lines even in the same patient. It is suggested that these genes are involved with the phenotypic characteristics and development of cervical carcinoma.
