Effects of Astragalus polysaccharide on gene expression profiles in islets of NOD mice with microarray technique
- VernacularTitle:以基因芯片技术研究黄芪多糖对NOD小鼠胰岛基因表达的影响
- Author:
Wei CHEN
;
Maohua YU
- Publication Type:Journal Article
- Keywords:
Astragalin;
Diabetes mellitus, type 1;
Immunology;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further investigate the molecular mechanism underlying the preventive effect of Astragalus polysaccharide (APS) on type 1 diabetes mellitus (DM) in NOD mice. Methods In order to explore the gene expression profiles of NOD mice′s islet cells treated with APS, cDNA microarray technique was applied. Results In APS group 5.47% gene expressions were obviously changed, 28 genes up-regulated, 35 genes down-regulated, and of which, 17 genes were functionally related to immunity. Conclusion The preventive effect of APS on type 1 diabetes in NOD mice is probably related to the action of APS in correcting the imbalance of Th 1/Th 2 cytokines.
