Recombinant human glucagon-like peptide-1 (7-36) enhances insulin release and insulin mRNA expression in INS-1 cells
- VernacularTitle:重组人胰高血糖素样肽1(7-36)促进INS-1细胞胰岛素释放与合成
- Author:
Qiuhong GONG
;
Jinning LOU
;
Liya YE
;
Guangwei LI
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide-1;
INS-1 cell;
Insulin;
Diabetes mellitus
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In INS-1 cells, the insulin secretion was investigated by radioimmunoassay (RIA) after 4 h incubation in medium containing different concentrations of glucose and recombinant human glucagon-like peptide-1 (rhGLP-1) (7-36). Insulin mRNA level in INS-1 cells was assessed by a semi-quantitative RT-PCR method. rhGLP-1 (7-36) is not only a powerful insulin secretagogue, but also can increase insulin gene expression in INS-1 cells.
