Peripheral nerve regeneration in response to target muscle injection with Methycobal
- VernacularTitle:靶肌肉注射甲钴胺对大鼠周围神经损伤再生的作用
- Author:
Yaojun LU
;
Yunkun ZHANG
;
Guangxiang HONG
- Publication Type:Journal Article
- Keywords:
Transcobalamins;
Peripheral nerves;
Administration, topical;
Nerve regeneration
- From:
Chinese Journal of Trauma
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of target muscle injection with different dosages of Methycobal in the sciatic nerve regeneration of adult rats so as to determine a better method and proper dosage of Methycobal for clinical treatment of peripheral nerve injury. Methods A total of 30 healthy Wistar rats were employed to make sciatic nerve injury model through immediate suture of the adventitia posterior to transection of the left sciatic nerve. The rats were divided randomly into three groups, ie, high dosage group (Methycobal for 300 ?g/kg, Group A), low dosage group (Methycobal for 100 ?g/kg, Group B) and control group (1 ml normal saline once a day, Group C),10 rats in each group. At 4 and 8 weeks, 5 rats from each group were taken to observe the effect of different dosages of Methycobal on rat sciatic nerve regeneration, where the detection indices included morphological changes and image analysis of sciatic nerves by optic and electron microscopic investigations as well as tricipital muscle wet weight. Results Four weeks after operation, tricipital muscle wet weight was (1.367?0.012) g in the Group A, (1.164? 0.011) gin the Group B and (0.950?0.009) g in the Group C, with significant difference among groups A, B and C (P
