Experimental study of intracellular ionized calcium level and oxidative stress during renal ischemic reperfusion injury
- VernacularTitle:缺血再灌注对肾细胞内钙水平和细胞凋亡的影响
- Author:
Yaowen FU
;
Wenlan ZHANG
;
Lijuan XUE
- Publication Type:Journal Article
- Keywords:
Kidney;
Ischemic reperfusion;
Calcium;
Apoptosis
- From:
Chinese Journal of Urology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of renal ischemic reperfusion injury on intracellular ionized calcium level and apoptosis. Methods The model of rat's acute renal ischemic reperfusion injury was established.A total of 30 Wistar rats were divided into 5 groups:control, 1 h ischemia and 1 h,2 h,24 h reperfusion groups (n=6 for each group).Renal intracellular [Ca 2+]i level was determined by Fura-2/AM fluorescence assay,and renal cell apoptosis rates were measured by flow cytometry. Results The renal intracellular [Ca 2+]i levels at 1h,2h and 24h timepoint of reperfusion were 156.2 nmol/L,181.6 nmol/L and 260.6 nmol/L,respectively,which were significantly higher (P
