Relationship between genetic diversity of surface protein of Streptococcus mutans and dental caries.Ⅱ Sequence of V-region and P-region gene of surface protein of Streptococcus mutans
- VernacularTitle:变形链球菌表面蛋白遗传多态性与龋病发生关系的研究Ⅱ表面蛋白V区、P区编码基因序列的测定
- Author:
Heng ZHUANG
;
Tianjia LIU
;
Deqin YANG
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
Membrane proteins;
Base sequence
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To sequence V-region and P-region gene of surface protein of serotype c Streptococcus mutans clinical isolates with different adherent abilities. Methods: The clinical isolates of serotype c S.mutans included two groups with different spaP-pv AluI genotypes, which were derived from previous studies in our lab. The genome DNA was extracted. The spaP-pv (2 060-3 157 bp) was amplified by PCR, then sequenced and analyzed. Results: Seven sites of AluI 5′-AG↓CT- 3′were included in the sequences of spaP-pv of both genotypes strains. The sequences of spaP-pv of ten a-genotype strains were the same except several site mutations, and so were those of b-genotype strains. Two different DNA fragments were revealed between a and b geotype strains. And they were located in the V-region of spaP. Conclusion: The mutation of gene encoding V-region of S.mutans clinical isolates might be related to the differences of adherent abilities .
