The Role of Fibronectin and Lectins Binding Receptors in Development of Neural Tube Defects Induced by Passive Smoking
- VernacularTitle:纤维粘连蛋白和凝集素受体在被动吸烟致神经管缺陷中的作用
- Author:
Dong WANG
;
Xiangmin YU
;
Ling LI
- Publication Type:Journal Article
- Keywords:
Neural tube defects;
Tobacco smoke pollution;
Fibronectins;
Lectins
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanisms of teratogenesis caused by passive smoking, temporospatial changes in surface carbohydrates of the embryonic neural epithelium and the mesenchyme around it were explored. Methods The pregnant golden hamsters were randomly divided into the treated group and the control groups. In the chamber made by ourself for passive smoking experiment, the animals of the treated group were exposed to smoke from embryonic day 4 to 7, 3 times per day for 4 consecutive days. At twelve o'clock and eighteen o'clock of embryonic day 8, eight o'clock of embryonic day 9 and eight o'clock of embryonic day 10 respectively, the pregnant golden hamsters of both treated group and control group were sacrificed, the uteri were removed and embedded routinely with paraffin, sectioned transversely in series. The sections of each group were stained with immunohistochemical ABC method and concannavalin agglutinin (ConA) and ulex europaeus agglutinin (UEA) were used as molecule probes to conduct the qualitative and quantitative analysis of the fibronectin and the related surface carbohydrates of the embryonic neural epithelium and the mesenchyme around it. Results During embryonic day 8 to10 of golden hamsters fibronectin reduced(F=30.168,P
