Effect of immunoregulation of IVIG to the expression of inhibitory Fc receptor
- VernacularTitle:大剂量免疫球蛋白对抑制性受体的免疫调控作用
- Author:
Aimin ZHANG
;
Junhua ZHENG
;
Taoyong CHEN
- Publication Type:Journal Article
- Keywords:
Immunoglobulins;
Antibodies;
Biphasic regulation
- From:
Chinese Journal of Organ Transplantation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of FcRIIa and FcRIIb on the surface of several immune cells.Methods The expression of FcRIIa and FcRIIb on the surface of several immune cells were detected by RT-PCR and Western blot before and after IVIG incubation. The difference of Fc?RIIb expression on U937 and human peripheral blood mononuclear cells (PBM) after IVIG induction. was also analyze by FACS.Results Under normal conditions Fc?RIIa was mainly detected in macrophages/monocytes and B lymphocyte cells, while Fc?RIIb could not be detected. After IVIG administration, the expression of FcRIIb on the surface of U937 cells was quickly up-regulated (RT-PCR). FACS analysis revealed that the Fc?RIIb expression on U937 and human peripheral blood mononuclear cells (PBM) after IVIG induction was also increased. Conclusion The effect of immunoregulation of IVIG was partly achieved by up-regulation of the expression of FcRIIb on the surface of some immune cells.
