Study on the immune tolerance induced by immature dendritic cells in coordination islet xenotransplantation
- VernacularTitle:未成熟树突状细胞诱导协调性异种胰岛移植耐受的研究
- Author:
Gang ZHAO
;
Fang WANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Transplantation conditioning;
Transplantation,heterologous;
Islets of langerhans
- From:
Chinese Journal of Organ Transplantation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inducible effect of immature dendritic cells (DC) on immune tolerance in coordination islet xenotransplantation.Methods Mature and immature DC were cultured from BALB/c mice with 20 ?g/L GM-CSF and 500 ?g/L GM-CSF+ 200 ?g/L IL-4, respectively, then loaded with major histocompatibility complex (MHC) antigen of Wistar rat. Mature and immature DC were injected into the mice with diabetes. After a week, the islets of Wistar rat or SD rat were transplanted under the envelop of left renal of the diabetes mice. The survival time of grafts was monitored. The immune reactivity of T cell was evaluated by mixed lymphocyte culture. Results Grafts in BALB/c mice pretreated with mature DC rejected quickly, while the survival time of grafts was significantly prolonged in the mice pretreated with immature DC. However, whether pretreated with mature DC or immature DC, grafts from SD rat were rejected soon. The splenocytes of tolerant mice showed mild proliferation. Conclusion The recipient pretreated with immature DC will lead to the anergy of T lymphocyte, and prolong the survival time of xeno-islet.
