Human p100 protein:a novel coactivator that enhances ST AT6-mediated gene transcriptional activation
- VernacularTitle:人p100蛋白:可增强STAT6基因转录活性的新因子
- Author:
Jie YANG
;
Zhi YAO
;
Chunyan YU
;
Silvennoinen OLLI
- Publication Type:Journal Article
- Keywords:
STAT6;
Human p100 protein;
RNA polymerase Ⅱ;
Gene transcription
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To setup a stable cell line which can overexpress human p100 protein,and then st udy the physiologic functions of p100 in regulation of STAT6-mediated gene tran scription.Methods:Using coimmunoprecipitation method to identify protein-protein interactions.STA T6-mediated gene transcriptional activation was detected by luciferase assay.Results:Human p100 protein interacted with both STAT6 and RNA polymerase Ⅱ,resulting in the enhancement of STAT6-mediated gene transcriptional activation.Conclusion:Human p100 protein acts as a bridging factor between STAT6 and basal transcripti onal machenary. [
