The detection of interleukin-2 receptor ?-chain gene mutation and protein expression of the tumor infiltrated Tcell in B-NHL
- VernacularTitle:B淋巴瘤中纯化TIL-T的IL-2R?基因突变及蛋白表达检测
- Author:
Jie LONG
;
Zulan SU
;
Qiuliang WU
;
Yong LIU
- Publication Type:Journal Article
- Keywords:
B-cell lymphoma;
Tumor infiltrating T cell(TIL-T);
IL-2R?
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the gene mutation,protein expression of IL-2R? of TIL-T and the pr oliferation of TIL-T with the present of rIL-2 in B-NHL. Methods:The gene mutation of IL-2R? was performed on 19 TIL-T by PCR-SSCP;proliferat ion assay of 17 TIL-T with the rIL-2 was tests by MTT;IL-2R? protein express ion in cryostat section of 29 B-NHL were determined by immunohistochemical stai n. Results:SSCP showed there is no mutation happened in the cDNA of IL-2R? of TIL-T.Pro liferation test showed the intensity of response of TIL-T was decreased in 76. 5% TIL-T(13 of 17 cases).The expression of CD25 protein in 86.2%(25 of 29 c ases) of B-NHL cases were (+) or (+/-). Conclusion:No genetic mutation had been found in IL-2R? of TIL-T,but IL-2R? protein i s weakly expressed in B-NHL;It indicated that there may be abnormal in the mech anism of activation of TIL-T by cell-cell contact. [
