Preparation of monoclonal antibodies against ?-amyloid peptide_(1-42)
- VernacularTitle:?淀粉样肽1~42单克隆抗体的制备
- Author:
Weijiang DONG
;
Haitao HU
;
Gaifeng FENG
;
Guangxiao YANG
;
Quanying WANG
- Publication Type:Journal Article
- Keywords:
A? 1-42;
monoclonal antibody (mAb);
f usion protein;
cell fusion
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a hybridoma secreting stab le monoclonal antibodies against ?-amyloid peptide (A? 1-42) with high titer. Methods By genetic engineering technology, A ? gene was recombined with the MIR of HBcAg to get the A? and HBcAg fusi on protein. Spleen cells from BALB/c mice immunized with A? and HBcAg f usion protein were fused with mouse myeloma cells SP2/0. Results Two strains of hybridomas (1H 7 and 1F 3) secreting stable monoclonal antibodies raised against A? 1-42 were ob tained. The subtypes of A? 1-42 antibodies were IgG 3. C onclusion The A? 1-42 monoclonal antibodies obtained have high titers and specificity.