Effects of serum on culturing sensory and motor neurons from a patient with acute motor axonal neuropathy
- VernacularTitle:急性运动轴索型神经病患者血清对体外培养感觉和运动神经元的影响
- Author:
Chunyan LI
;
Xueqin SONG
;
Liqin WANG
;
Xiaojuan WANG
- Publication Type:Journal Article
- Keywords:
Axons;
Motor neuron disease;
Gamglia, spinal;
Motor neurons;
Cells, cultured
- From:
Chinese Journal of Neurology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of serum from a patient with acute motor axonal neuropathy (AMAN) on the sensory and motor neurons culture in vitro from embryonic rats. Methods Dorsal root ganglions and spinal ventral tissue were isolated from embryonic rats and digested into dissociated cell suspenstion for culture. The cells were identified by immunohistochemistry stain. When culturing for a week, AMAN serum was exposed in a 25% concentration, with normal human serum as control group. The AMAN serum was tested by anti-Penner O∶19 campylobacter jejuni lipopolysaccharide antibody positive. The changes of motor and sensory neurons were observed and the neurons were stained by using Guillery Shirra and Webster method which was sensitive to degeneration of nerve fiber. Results After normal serum exposure, neurons and their neurites were normal and stained in yellow color without silver granular deposition by using Guillery Shirra and Webster method. While after AMAN serum exposure, the axon from motor neuron became degenerating and stained in brown-black color increased silver-phile property. Conclusion The serum of AMAN patient might be specifically toxic to the neurites of motor neuron and might cause the degeneration of axon following soma changes. The damage of axon might be the response of campylobacter jejuni lipopolysaccharide antibody without participation of macrophage and complements.
