Detection of Aneuploidy from Single Cells by Array-Comparative Genetic Hybridization
- VernacularTitle:SNP基因芯片结合多重置换扩增技术检测单细胞非整倍体的研究
- Author:
Jiawei LING
;
Cong FANG
;
Yanwen XU
;
Guanglun ZHUANG
- Publication Type:Journal Article
- Keywords:
SNP mapping array;
multiple displacement amplification;
aneuploidy screening;
preimplantation genetic diagnosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):468-472,476
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To set up an optimized protocol for aneuploidy detection from single cells through Array- Comparative Genetic Hybridization (CGH).[Method] Two cell lines,trisomy 18 (Tri-18;GM02732,47,XY,+18) and chromosome 4 segment deletion [sDel-4;GM00343,46,XY,4(del) (qter ＞ p14)],were used in the study.In combination of 10 k 2.0 SNP mapping array platform and multiple displacement amplification (MDA),the diagnostic accurate rates of MDA product from single cells of the two cell lines using gDNA and single-cell MDA product as reference were compared.[Result] An extremely lower call rate (3.2 ± 1.2)% in the negative control group was observed compared to the experiment groups.When the single-cell MDA product was used as reference,the standard deviations of Log2 (signal intensity ratio) were significantly decreased in both groups,compared with when the gDNA as reference (P ＝ 0.004).Through CNAT analytic software,some specific chromosomes (16,17,19,and 22) presented obvious preferential amplification (PA) when the gDNA was used as reference,but this PA could be eliminated when single-cell MDA product was used as reference.[Conclusion] 10 k 2.0 SNP mapping array in combination with MDA could be a quick,highly efficient and accurate method to detect aneuploidy in single cells.
