The role of cell apoptosis in glioma chemotherapy evaluation
- VernacularTitle:替尼泊苷和洛莫司汀诱导脑胶质瘤细胞凋亡的机制
- Author:
Rong XU
;
Yong HUA
;
Ping ZHONG
;
Qiwu XU
;
Xiang GAO
- Publication Type:Journal Article
- Keywords:
glioma;
apoptosis;
mitochondrial membrane potential;
cell cycle
- From:
Fudan University Journal of Medical Sciences
2009;36(6):692-695,706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of cell apoptosis during the chemotherapy of human gliocytoma in order to get effective suvilliance on the effect of chemotherapy. Methods Gliocytoma cells were isolated and cultured from 40 human gliocytoma samples. Mitochondrial membrance potential (MMP), cell cycle, the level of Bcl-2 and Bax were detected by flow cytometry (FCM) at 24, 48, 72 hours respectively of incubation with Lomustine (CCNU) and Teniposide (VM-26), and the trends were also analysed. Results MMP decreased greatly, the apoptosis part in the cell cycle ananlysis increase, the expression level of Bcl-2 decreased, and that of Bax increase rapidly, while the Bcl-2/Bax ratio decreased. Conclusions CCNU and VM-26 have singnificant effect in gliocytoma chemotherapy on inducing gliocytoma cell apoptosis. VM-26 has more stronger effect on the cell cycle. MMP is the most sensitive and the fastest index in apoptosis detection.
