Researeh on resveratrol's effect in suppressing growth and inducing apoptosis of lens epithelial cells
- VernacularTitle:白藜芦醇抑制晶体上皮细胞生长与诱导细胞凋亡的实验研究
- Author:
Qin FENG
;
Qiang WU
;
Bin LU
;
Qinjin CHEN
- Publication Type:Journal Article
- Keywords:
Resveratorl;
Cataract;
Epithelial cells;
Apoptosis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(2):264-266,后插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the contribution of resveratrol(Res)to the proliferation and apoptosis of lens epithelial cells in vitro.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to measure its effects on the proliferation of lens epithelial cells cultured with different concentrations of Res for 24h,48h and 72 h.HE staining,transmission electron microscope(TEM),TUNEL fluorescence staining and the Annexin V assay by flow cytometer(FCM)were applied to observe the ceumorphological change detect the apoptosis induced by Res quantitatively.Results Res obviously suppressed the proliferation(P＜0.01)and induced the apoptosis of lens epithelial cells in concentration-dependent manner.The rate of apoptosis in control was 4.67%,and 17.31%,32.77%after having been treated with 100μmol/L and 200μmol/L Res for 24h,respectively.Conclusion The results of this study confirm the ability of Res to suppress the growth and the proliferation of lens epithelial cells with a typical apoptotm feature in vitro.Therefore,Res might be considered as a possible treatment strategy for after-cataract.
