The protective effect of sodium hyaluronate on the cartilage of rabbit osteoarthritis and its effects on PPAR-gamma mRNA expression
10.3760/cma.j.issn.1007-7480.2009.02.014
- VernacularTitle:透明质酸钠关节腔内注射对兔软骨的保护作用及对过氧化物酶体增殖物激活受体γ mRNA表达的影响
- Author:
Jianlin ZHOU
;
Shiqing LIU
;
Bo QIU
;
Hao PENG
;
Jianghua MING
- Publication Type:Journal Article
- Keywords:
Sodium hyaluronate;
Osteoarthritis;
Cartilage;
Peroxisome proliferators-activated receptor gamma
- From:
Chinese Journal of Rheumatology
2009;13(2):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of sodium hyaluronate (Na-HA),and its effects on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-γ) in cartilage of rabbit osteoarthritis (OA) model.Methods Forty eight white rabbits were divided into A,B,C groups randomly.Group A were normal controls,groups B and C were underwent unilateral anterior cruciate ligament transection (ACLT).The rabbits in group B were injected normal saline after ACLT;Group C rabbits received intra-articular 1% sodium hyaluronate (HA) injections 5 weeks after surgery,0.3 ml once a week.At week 11 after the surgery,all rabbits were sacrificed.The cartilage changes on the medial femoral condyles were graded.Cartilage sections were stained with safranin-O and HE,mRNA expression of PPAR-γ was detected by real time polymerase chain reaction (Real Time-PCR).Results Cartilage degeneration in group B was significantly more severe than in groups A and C.The grey value of Safranin-O of B group were higher than groups A and C.Expression of PPAR-γ mRNA in group B was higher than that in groups A and C.Conclusion NaHA has a protective effect on articular cartilage degeneration,and the inhibitory effect on PPAR-γ mRNA expression may be one of the therapeutic mechanism of Na-HA.
