A study on production of Th-derived cytokins by PBMC stimulated with PHA treated with Anti-CD86 mAb in children with asthma
- VernacularTitle:Anti-CD86mAb干预哮喘患儿PBMC后对其产生Th源细胞因子影响的研究
- Author:
Xiaoping ZHU
;
Xiqiang YANG
;
Zhou FU
;
Liping JIANG
;
Jiali WANG
- Publication Type:Journal Article
- Keywords:
Asthma;
IL-4;
IL-13;
CD86mAb
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of T lymphocytes activation co-stimulation pathway in asthma pathogenesis and the ability of therapy asthma with Anti-CD86mAb.Methods:The blood samples were taken from 28 asthma children( including 18 male and 10 female, age 1 year-8.08 years) and 15 normal children( including 7 male and 8 female, age 3.25 years-10 years).ELISA was used to detect the levels of IL-4、IL-13、IFN-? in culture supernatants of PBMC stimulated with PHA and treated with mouse anti human CD86mAb. Results:①When treated control PBMC with anti-CD86mAb, the level of IL-4 in control group(13.30?4.66 pg/ml) was lower than that of mouse IgG control group (15.20?5.22 pg/ml,P
