Study of the panel of 6 microsatellites in diagnosis of bladder cancer
- VernacularTitle:六位点微卫星组合用于膀胱癌诊断的研究
- Author:
Jun ZHAO
;
Dalin HE
;
Lin YANG
- Publication Type:Journal Article
- Keywords:
Bladder cancer;
Microsatellite instability;
Lost of heterozygosity
- From:
Chinese Journal of Urology
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of the panel of 6 microsatellites in detection of bladder cancer. Methods In the tumor tissue and urine sediment of 32 cases of bladder cancer 10 microsatellites were chosen and PCR-SSLP silver staining assay was conducted according to the methods described in the literature and our previous study.15 cases of non-bladder cancer served as controls. Results Microsatellite alternate (MA) was found in 30 out of 32 cases of bladder cancers,the sensitivity being 93.8%.The MA of urine sediment of 15 cases of non-bladder cancer was negative,the specifity being 100.0%.Among the 10 microsatellites, 6 ones were chosen;the MA positivity of the panel of the 6 ones was 90.6% (29/32).This result was not significantly different from that of the panel of 10 microsatellites. Conclusions MA assay is a sensitive,effective method for detection of bladder cancer.Compared with the panel of 10 microsatellites,the panel of 6 microsatellites may be a better tool for detection of bladder cancer.
