The expression of chicken interleukin 2 in Pichia yeast
- VernacularTitle:鸡白细胞介素2在毕赤酵母细胞中的表达
- Author:
Jian XU
;
Yongjun FAN
;
Long LI
;
Wangjun WAN
;
Jianrong LI
;
Lian YU
- Publication Type:Journal Article
- Keywords:
chicken IL-2;
pPIC9;
Pichia yeast;
reagent of immune enhanced
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express recombinant chicken interleukin 2 (IL-2) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL-2. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL-2 gene was amplified and cloned into pPIC9. The pPIC9-IL-2 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Results The protein was examined with SDS-PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL-2 has been expressed in Pichia yeast.
