Investigations of synchronization of expression between apoptosis and P27~(Kip1),PCNA in esophagus squamous cell carcinoma
- VernacularTitle:食管鳞状细胞癌组织中细胞凋亡与P27~(Kip1)、PCNA同步表达的关系
- Author:
Yimin XIONG
;
Hualing XU
;
Yiming WANG
- Publication Type:Journal Article
- Keywords:
esophagus squamous cell carcinoma;
P27Kip1;
proliferating cell nuclear antigen (PCNA);
apoptosis
- From:
China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the mutual relationships between apoptosis,cell proliferation correlative gene PCNA and tumor suppressor gene P27 Kip1 as well as to investigate if they have something to do with the occurrence,development,and prognosis in esophagus squamous cell carcinoma.Methods:47 cases of primary esophagus squamous cell carcinoma were put into investigative group whose tumor samples from operation resection were studied. 20 cases with normal esophagus cell mucous membrane tissue were put into the control group. Expression level of PCNA,P27 Kip1 was detected by immunohistochemistry method (S-ABC) in both groups. Apoptotic index was detected by TdT mediated deoxyuridylic acid(dUMP)nicking end labeling (TUNEL method) in both groups.Results:①In the group of 47 cases of esophagus squamous cell carcinoma,there were 17 cases of positive expression in P27 Kip1 ,and the positive rate was 36.17% , average proliferation index(PI) was 45.86?19.11%,average apoptotic index is 1.05%?0.59%,ratio of PI/AI is 57.37? 45.17 ,all of which were statistically significant for difference between normal tissue and carcinoma in esophagus squamous cell (P
