Effect of 1,2:5,6-dianhydro-3,4-diacetylgalactitol and p53 gene transfection for the growth of hepatocellular carcinoma
- VernacularTitle:二乙酰去水卫矛醇联合p53基因转染对肝癌细胞生长的影响
- Author:
Xiangjin LAI
;
Linzhi CAO
;
Ying ZHOU
- Publication Type:Journal Article
- Keywords:
p53 gene;
hepatocellular carcinoma;
1,2:5,6-dianhydro-3,4-diacetylgalactitol
- From:
China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the effect of p53 gene transfection and 1,2∶5,6-dianhydro-3,4-diacetylgalactitol (DADAG) on the growth of hepatocellular carcinoma(HCC). Methods:The HCC line HLE that were transfected with plasmid pUHD10-3 recombined with wt-p53 gene by lipofectamine-mediated were cultured in medium containing DADAG. 3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid(MTT) technique was used to analyse the growth of HCC cells. Results:The growth of HCC cell line HLE were inhibited after being transfected with wt-p53 gene,and 67.68% of the cells were inhibited on the fourth day. 53.09% of HCC cell line HLE were inhibited when they were exposed to DADAG. When the HCC cell line HLE transfected with wt-p53 gene were exposed to DADAG,85.37% of the cells were inhibited. Conclusions:The inhibition efficiency of HCC was enhanced by the combination of DADAG and wt-p53 gene transfection.
