HER-2/neu gene amplification and expression in nasopharyngeal carcinoma and their clinical significance
- VernacularTitle:鼻咽癌患者HER-2/neu基因扩增和表达及其临床意义
- Author:
Kangsheng GU
;
Zhongzhen GUAN
;
Yan FANG
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma;
HER-2/neu gene;
gene amplification;
gene expression
- From:China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study HER-2/neu gene amplification and expression in nasopharyngeal carcinoma (NPC) and their clinical significance.Methods:HER-2/neu gene amplification and expression in NPC tissues were detected with fluorescence in situ hybridization (FISH,Vysis PathVysion TM kit) and reverse transcription polymerase chain reaction (RT-PCR) and immounhistochemistry (IHC,DAKO Herceptin Test TM kit).Results:No HER-2/neu gene amplification but gene overexpression was detected in NPC. HER-2/neu overexpression was caused by mRNA overexpression.Conclusions:HER-2/neu gene has not been amplified,but overexpressed,thus HER-2/neu gene overexpression did not show prognostic significance in NPC.
