Experimental study on inducing bone marrow stromal cells into Schwann-liked cells in vitro by modified method
- VernacularTitle:改良法体外诱导骨髓基质干细胞分化为类许旺细胞
- Author:
Jianyun WANG
;
Xiaolin LIU
;
Jiakai ZHU
;
Yubin DENG
;
Jianping XIANG
;
Fobao LI
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Schwann-liked cells;
MTT method;
Flow cytometry
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for a more efficient method to induce bone marrow stromal cells (BMSCs) into Schwann-liked cells (SLCs) in vitro. Methods On the base of the method which was used by Dezawa (it was looked as the traditional inducing method),some steps were modified,namely,to use all the reagents in half dose in the same time and two times intervally,this method was looked as the modified inducing method.After induced by the two methods,the cells morphologic characteristic,the positive ratio of immunocytochemical dye with anti-S-100 and anti-GFAP,the cells activity measured by MTT method and the DNA percentage in S period measured by flow cytometry were compared with each other respectively to evaluate the methods'effects. Results Compared with the traditional method,the cells induced by the modified method were more similar to the primary Schwann cells in morphology,more positive proportion in immunocytochemic dye with anti-S-100 and anti-GFAP,less damage in the activity and more percentage in S period. Conclusion The modified method had more advantages such as less damage on cells and more efficiency in inducing BMSCs into SLCs.
