Effects of monoclonal antibody of insulin-like growth factor-Ⅰ receptor on proliferation and apoptosis of papillary thyroid carcinoma cells
- VernacularTitle:IGF-Ⅰ受体单克隆抗体对甲状腺乳头状癌细胞增殖及凋亡的影响
- Author:
Jun SHEN
;
Maohua YU
;
Weihua CHEN
- Publication Type:Journal Article
- Keywords:
Papillary thyroid carcinoma cells;
Receptor, insulin-like growth factor-Ⅰ;
Antibodies, monoclonal;
Proliferation;
Apoptosis
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of monoclonal antibody of insulin-like growth factor Ⅰ receptor (IGF-ⅠR) in the proliferation and apoptosis of papillary thyroid carcinoma cell line CGTH W-3, and the effect of IGF-ⅠR monoclonal antibody on papillary thyroid carcinoma cells and normal follicular cells in primary culture. Methods MTT and TUNEL method were used to determine the proliferation and apoptosis of cells respectively after IGF-ⅠR monoclonal antibody intervention. Results The inhibitory effect on cell proliferation was enhanced with the increasing IGF-ⅠR monoclonal antibody concentration and duration of action. IGF-ⅠR monoclonal antibody induced apoptosis of CGTH W-3 cells remarkably even in the concentration of 50 nmol/L. Primary papillary thyroid carcinoma cells and normal follicular cells were inhibited by 100 nmol/L IGF-ⅠR monoclonal antibody, and the degree of inhibition was significantly higher in carcinoma cells than that in normal follicular cells (P
