Effect of LIF gene modified ECV-304 cells on the ex vivo culture of HSC/HPC in cord blood
- VernacularTitle:LIF基因修饰的ECV-304细胞对脐血造血细胞体外培养的影响
- Author:
Xin YU
;
Li MIAO
;
Jingcheng MIAO
- Publication Type:Journal Article
- Keywords:
human LIF Eukaryotic;
Expression;
adhesion molecule;
L-selectin;
CD34+ cell
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the ECV-304 cells modified with LIF gene on the ex vivo culture of HSC/HPC in cord blood.Methods The ECV-304 cells were infected by Eukaryotic Expression plasmid pcDNA3.0LIF,and the positive ECV-304 cells were obtained by selected with G418.These cells were used to co-culture with CD34+ cells of cord blood.The phenotype of CD34+ and CD34+ CD54+、CD34+ CD62L+ primitive progenitors was detected by flow cytometry.Results The LIF gene can express in ECV-304 cells steadily.ECV-304 cells modified with LIF gene can improve expansion of CB CD34+、CD34+CD54+ and CD34+ CD62L+ cells while sustaining the expression of homing-related adhesion molecule.Conclusion The ECV-304 cells modified with LIF gene can not only significantly expand CB hematopoietic progenitor cells ex vivo,but the expanded CD34+ cells may well retain their homing ability.
