The effects of presenilin-1 mutation on the proliferation and apoptosis of all-trans retinoic acid(RA) -induced PC12 cells
- VernacularTitle:Presenilin-1突变对全反式维甲酸诱导的PC12细胞生长和凋亡的影响
- Author:
Xiaohua TENG
;
Qilin HUANG
;
Kecheng ZHANG
- Publication Type:Journal Article
- Keywords:
Mutation;
Apoptosis;
Alzheimer disease
- From:
Chinese Journal of Geriatrics
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mutated PS-1 on the proliferation and apoptosis of RA-induced PC12 cells . Methods The MTT assay, flow cytometry, TUNEL method were used in the research. Results An inhibition of proliferation was demonstrated in the RA-induuced PC12 cells expressing PS-1 mutant L286V. The proliferation of the cells was blocked in the G1→S phase. Whether with or without fetal bovine serum(FBS), PS-1 mutant L286V was able to accelerate the apoptosis of RA-induced PC12 cells. This action was enhanced markedly under the culture without FBS. Conclusions The RA-induced PC12 cells expressing PS-1 mutant L286V shows that the proliferation is inhibited and blocked in the G1→S phase. Whether with or without FBS, PS-1 mutant L286V can accelerate the apoptosis of RA-induced PC12 cells. This action is enhanced markedly under the culture without FBS.
