Expression of vascular endothelial growth factor in proliferative maxillo-facial hemangioma of infant
- VernacularTitle:婴幼儿颌面部增生期血管瘤中VEGFR及内皮抑素的半定量RT-PCR分析比较
- Author:
Gang CHEN
;
Ligang LIU
;
Shengnan YAN
- Publication Type:Journal Article
- Keywords:
VEGF;
VEGFR;
Hemangioma;
Endostatin;
Reverse transcriptase-polymerase chain reaction, RT-PCR
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relative quantities of flt-1, KDR, endostatin mRNA in tissue specimens of human hemangioma and great saphfenous vein with the modified reverse transcriptase-polymerase chain reaction (RT-PCR). Methods Total RNA from human specimens by Trizol was reverse-transcribed and amplified by PCR in 4 deffirent tubes containing one of the primer pairs such as flt-1, KDR, endostatin or ?-actin. The target genes and beta-actin PCR products were about 200 bp in length. The ratio of the yield of the target gene PCR product to the beta-actin PCR product could be calculated after 35 cycles of amplification.Results These ratios were correlated positively ( P=0.014,P=0.019) in two groups, but no relationship in endostatine level was observed (P=0.436).Conclusions VEGFR may play a very important role in the pathogenesis of the congenital hemangioma in children. But the endostatin may take little effect on it.
