Effect of Static Magnetic Field on Growth of Rat Embryonic Spinal Cord Neurons in Vitro
- VernacularTitle:稳恒磁场对大鼠胚胎脊髓神经细胞发育的影响
- Author:
Lirong DUAN
;
Zhijian ZHANG
;
Quanyi WU
- Publication Type:Journal Article
- Keywords:
Neurons;
Cell differentiation;
Lipid peroxidation;
Magnetic field
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of static magnetic field (SMF) on development of rat embryonic spinal cord neurons. Methods Primary cultured embryonic spinal cord neurons of Wistar rat were exposed to 1.0, 10.0, 50.0, 100.0 and 200.0 mT static magnetic field. The morphological structure, cell's differentiation and proliferation of the embryonic spinal cord neurons were observed and the contents of MDA, superoxide dismutase (SOD) and protein contents in the neurons were determined. Results Static magnetic field at density of 50-200.0 mT could inhibit the differentiation and proliferation of the cells and the phenomena such as cell aggregation, detouchment of some cells, decrease of clone-formation rate and the size of the cells were observed. The contents of MDA in the cells were increased, while the activities of SOD and the level of protein were decreased. Conclusion Static magnetic field might damage the development of embryonic spinal cord neurons by enhancing the lipid peroxidation.
