Crabtree Mary B: Application of RT - PCR in identifying genome of several viruses causing acute encephalitis syndrome in Vietnam
- Author:
Pham Thi Nga
- Publication Type:Journal Article
- Keywords:
encephalitis syndrome, RT – PCR
- MeSH:
Reverse Transcriptase Polymerase Chain Reaction;
genome;
viruses;
Encephalitis
- From:Journal of Preventive Medicine
2004;14(6):5-10
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
RT- PCR technique with degenerative primers of three viral groups was used to select cerebrospinal (CSF) samples for isolating of mosquito borne viruses causing acute encephalitis (AES). The study results showed that 16 of 53 samples were confirmed with positive by RT-PCR as following: 8/53 (15.09%) were positive with primers of mosquito borne viruses; 7/53 (12.96%) positive with primers of latent viruses, and 1/53 (1.88%) positive with viral group of hand-food-mouth diseases. RT-PCR technique cannot replace virus isolation, but it orients the relevant selection of CSF samples for virus isolation. The Aedes albopictus clone C6/36 cells were used to isolate. There were 7 isolated virus strains, 5 of them were Japanese encephalitis virus, one of them belonged to a new type Arbovirus group in Vietnam and 1 strain was unknown
