Analyse on polymorphism of endothelial nitric oxide synthase gene(CA)_n in type 2 diabetes mellitus patients
- VernacularTitle:2型糖尿病患者内皮型一氧化氮合酶基因(CA)_n多态性分析
- Author:
Fengjin GUO
;
Huiming PENG
;
Zheng ZHANG
;
Ning YAN
- Publication Type:Journal Article
- Keywords:
Nitric-oxide synthase;
Diabetes mellitus,type 2;
Polymorphism,restriction fragment length;
Gene frequency
- From:
Chinese Journal of Geriatrics
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of the (CA)n dinucleotide repeats polymorphism of the eNOS gene with type 2 diabete mellitus (DM) and the serum NO level in DM patients. Methods The eNOS genotype was assessed in 179 unrelated Chinese of Han nationality,including 93 patients with DM and 86 normal controls(NC).Genotype was determined by polymerase chain reaction(PCR) and denature polyacrylamide gel electrophoresis (PAGE) with silver stain.Fasting serum NO level was measured by nitrate reductase and fasting serum NOS level was measured by spectrophotometer. Results Twenty two different alleles were identified containing 19-40 CA repeats,in which the frequency of (CA) 30 (166bp)was the highest. The heterozygosity (H) and polymorphism information content (PIC) were 0.85 and 0.83 respectively. Frequency of allele (CA) 34 was higher in DM (7.5%) than in NC (2.9%) significantly. Compared with NC,the serum NO level was significantly lower in T2DM with genotype (CA)n 1 (CA)n 2 when n 1≥34 or n 2≥34. Conclusions The allele(CA) 34 is associated with the susceptibility to DM and may be involved in the development of DM. The eNOS gene polymorphism is connected with the serum NO level.
