Establishment of a human large cell lung cancer multi-drug resistance cell line H460/cDDP and its biological characteristics
- VernacularTitle:人大细胞肺癌多药耐药株H460/cDDP的建立及其生物学特性
- Author:
Tao WANG
;
Jun XU
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
lung neoplasm;
large cell lung cancer cell Multi-drug resistance;
cisplatine;
cultured tumor cells
- From:China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To establish a human large cell lung cancer multi-drug resistance cell line H460/cDDP and explore its biological characteristics. Methods:A resistant human larget cell lung cancer cell line (H460/cDDP) was established by intermittent high dose cisplatin selection from the parental cell line H460. Drug sensitivity was detected by MTT assay. The changes of its biological characteristics were determined using light microscopy, Trypan Blue staining rejection, cell counting, chromatosome analysis; Neoplasia formation test in nude mouse was performed to investigate its in vivo characteristic. Results:H460/cDDP cell line was developed after about 6 months and the resistance index to cisplatin was 10.21. H460/cDDP cells exhibited cross-resistance to 5-Fuorouracil, adriamycn, etoposide and methotrexate. Compared with the parent cells, the morphology wac changed; doubling time prolonged (from 20.78h to 36.46h), while the chromatosome number and caryotype were similar. After being frozen, deposited and resuscitated repeatedly, its biological characteristics remained stable. It had neoplasia formation ability in vivo, but the forming time was longer than its parental cell. Conclusions:The newly established multi-drug resistant large cell lung cancer cell line H460/cDDP cell line possessed the typical multi-drug resistant phenotype. It was stable and had neoplasia formation ability in vivo,suitable for research.
