Influence of doxorubicin chemotherapy on the expression of epithelial growth factor in the Balb/c mouse hepatocarcinoma
- VernacularTitle:荷瘤小鼠多柔比星化疗后表皮生长因子(EGF)表达的检测
- Author:
Chunbao GUO
;
Feng CHEN
;
Xianqing JIN
- Publication Type:Journal Article
- Keywords:
cancer animal model;
epithelial growth factor;
doxorubicin;
RT-PCR;
enzyme linked immunosorbent assay(ELISA);
cultured tumor cells
- From:China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the influence of doxorubicin chemotherapy on overexpression of epithelial growth factor. Methods:Cancer animal model was made and treated with doxorubicin. The concentration of EGF protein in Plasma and mRNA in cancer tissue were detected by ELISA and Real-time quantitative RT-PCR respectively and the correlation of this parameter was evaluated. Results:The plasma concentrations of EGF expression were 3X Group 1.19?0.42 pg/ml, 1X Group 1.61?0.51 pg/ml, control group 2.13?0.68 pg/ml, and normal 0.91?0.33 pg/ml respectively. The concentration of EGF were analysed by ANOVA q test. The median concentrations of mRNA were 3X Group 1.6?10 3 copies/ml, 1X Group 8.5?10 4 copies/ml, control 4?10 5 copies/ml, and normal 2.5?10 2 copies/ml respectively, and were analysed by rank-sum test. It was shown that the control group had higher EGF and EGFR expression level than those treated with doxorubicin. EGF and EGFR expression of 3X Group were lower than that of 1X Group but remained higher than that of normal (P
