The impact of cell period and cell surface antigen in cervical cancer cell lines by interferon-? gene transfer
- VernacularTitle:?-干扰素基因转染对宫颈癌细胞株细胞周期及表面抗原的影响
- Author:
Lei ZHANG
;
Qiang DING
;
Dehua XU
- Publication Type:Journal Article
- Keywords:
interferon-?;
gene therapy;
cervical cancer;
surface antigen;
cell period;
apoptosis;
cultured tumor cells
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To explore the tumor cell responses after IFN-? gene transfer by analyzing the expression of cell surface antigen,apoptosis and the impact of cell period. Methods:We constructed IFN-? gene expression vector-pcDNA3- IFN-?, and transfected it into HeLa cell lines by Lipofectamine. The expression of IFN-??the Human Leukocyte antigen(HLA-DR) after gene transfer were measured. The apoptosis of tumor cells and the variety of cell period were also measured to investigate the anti-tumor mechanism by IFN-? gene therapy. Results:The results showed that the HLA-DR expression in Hela cell lines increased significantly(P
