The expression of E-cadherin and its relation with invasive ability of basal membrane in tumor cells
- VernacularTitle:肿瘤细胞E-cadherin的表达与对基底膜浸润能力的关系
- Author:
Xiangming CHE
;
Shufeng WANG
;
Kang WANG
;
Wei SHENG
;
Xiaoyi LIU
- Publication Type:Journal Article
- Keywords:
E-cadherin;
basal membrane;
invasive ability
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of down-regulation of E-cadherin on the invasive ability of tumor cells. Methods Human pancreatic carcinoma cell lines JHP-1, PANC-1, and MIA PaCa-2 were selected. The immunocytochemistry, Western blot and invasive-MTT assay were used to observe the expression and the contents of E-cadherin in the tumor cells. Results Immunocytochemistry showed that E-cadherin expression was on cell membrane. The expressions of E-cadherin were preserved in JHP-1, reduced in PANC-1, and showed negative in MIA PaCa-2. Western blot analysis showed that the protein content was the highest in JHP-1 and lowest in MIA PaCa-2. According to invasive MTT assay, the invasive ability of MIA PaCa-2 was the strongest, followed by PANC-1 and JHP-1 (P
