Study on Partial Purification of the Native Human Gamma Interferon (HuIFN-?)
- VernacularTitle:制备部分纯化人白细胞?-干扰素方法的探讨
- Author:
Naiqing BAI
- Publication Type:Journal Article
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
A method for partially purifying the native human gamma interferon (HuIFN-?) was presented. The rude HuIFN-?was concentrated by PEG 4 000, precipitated by (NH_4)_2SO_4 and purified by CM-Sephades C-50(more than 100 times). The titre of HuIFN-?was 1.3X10~5u/ml and its specific activity was 1. 0 X 10~5u/mg protien. Quality control tests were performed and 10 volunteers received intramuscularly the purified HuIFN-?without significant changes in body temperature, pulse rate and blood pressure. It is considered to be suitable for clinical trial.