THE EXPRESSION OF CYCLIN DEPENDENT KINASE 11 FOLLOWING TRANSECTED SPINAL CORD INJURY IN RATS
- VernacularTitle:细胞周期蛋白依赖性蛋白激酶11在大鼠脊髓横断性损伤后的表达变化
- Author:
Feng XIAO
- Publication Type:Journal Article
- Keywords:
Cyclin dependent kinase 11;
Transected spinal cord injury;
Immunohistochemistry;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To explore the expression and distribution of cyclin dependent kinase 11(CDK11) after transected spinal cord injury(tSCI). Methods tSCI was performed at T9 in adult Sprague-Dawley(SD) rats.The rats were sacrificed 1day,3days,5days,7days,14days after operation respectively.Western blotting was used to detect the changes of CDK11 protein expression after tSCI,and the distribution and localization of CDK11 in spinal cord were investigated by immunohistochemical staining and immunofluorescence double staining. Results Western blotting analysis showed the expressions of CDK11~(p58) and CDK11~(p110),which were the two major isoforms of CDK11,increased at first and then had a decrease tendency in both broken ends of the transected spinal cord.CDK11~(p58) was enhanced on the 3rd day and remained high level until the 7th day; while CDK11~(p110) was also increased,on the 3rd day but went down rapidly after the 5th day.Immunohistochemistry staining was indicated that CDK11 was distributed evenly in the normal spinal cord.On the 3rd day,the staining was elevated obviously in both white and grey matter.Immunofluorescence double staining suggested that CDK11 was colocalized with neuronal neclei(NeuN),cyclic nucleotide 3′phosphohydrolase(CNPase) and glial fibrillary acidic protein(GFAP).Conclusion The expression of CDK11 undergoes spatiotemporal changes after tSCI.These alterations may be involved in secondary spinal cord lesion such as neuronal apoptosis and proliferation of glial cells.
