Association of aging with gene OPG, ODF expressions in human osteoblasts in vitro
- VernacularTitle:体外培养人成骨细胞OPG、ODF基因表达与增龄相关
- Author:
Mingxiang YU
;
Hongfu WANG
;
Weifang JIN
;
Shuzhu GU
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Cell culture;
Gene expression;
Aging
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Human osteoblasts from donors of different age (≤5, 30-40, 50-60, ≥70) groups were isolated and cultured. Osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) mRNA expressions were assayed by RT-PCR when the osteoblasts in the second passage were cultured for 14 days. Results showed that human osteoblasts from different age groups expressed OPG and ODF mRNA. Quantity of OPG mRNA and ODF mRNA expressions was correlated to donor′s age.
