Expression of human proinsulin gene in non-?-cells by point mutation with concise PCR
- VernacularTitle:简捷的PCR点突变实现人胰岛素原基因非?细胞表达
- Author:
Fengshan YUAN
;
Lixin YANG
;
Kun ZHANG
;
Dahui WANG
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Point mutation;
Gene, proinsulin;
Gene expression
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The human proinsulin gene was introduced a cleanage site for furin with two point mutation by polymerase chain reaction. The mutated gene was subcloned to the retroviral vector, and transferred into HepG2 hepatocarcinoma cells. Mature insulin can be expressed and secreted into supernatant by transferred HepG2 cells. The site mutation method is concise and with no misproduction.
