EXPRESSION OF TRANSFORMING GROWTH FACTOR-?1 AND ITS SIGNAL TRANSDUCER SMAD2 AND SMAD4 IN ALPACA TESTIS
- VernacularTitle:转化生长因子-?1及其信号传导分子Smad2与Smad4在羊驼睾丸的表达
- Author:
Changsheng DONG
- Publication Type:Journal Article
- Keywords:
Testis;
TGF-?1;
Smad;
Smad4;
Western blotting;
Immunohistochemistry;
Alpaca
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and localization of TGF?1 and its signal transducer Smad2 and Smad4 which play important roles during testicular development and spermatogenesis in alpaca testis. Methods The whole testes were obtained from alpacas aged 24 months(n=3) at the Scientific Research Base of Shanxi Agriculture University.The protein expressions of TGF-?1、smad2、smad4 in alpaca testes were examined by Western blotting and SABC. Results The distributions of(TGF-?1)、Smad2 and Smad4 in the testes of alpacas aged 24 months were demonstrated.Conclusion Our findings show that TGF-?1、Smad2 and Smad4 are involved in the regulation of spermatogenesis,and provide direct evidences for the moleculer mechnism of TGF-?1 action in the alpaca testis during spermatogenesis.
