The expression, purification and identification of recombinant SARS S1 subunit expressed in E. Coli
- VernacularTitle:SARS病毒S1蛋白的克隆、表达及其重组蛋白免疫学特性的鉴定
- Author:
Yan LI
;
Xiaoang YANG
;
Xueyuan DONG
;
Yuedan WANG
;
Weifeng CHEN
- Publication Type:Journal Article
- Keywords:
SARS;
Coronavirus;
Protein S
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the course and mechanism of the immune response to SARS virus. Methods:The recombinant SARS virus S1 subunit was expressed in E. Coli according to the results of bioinformatics analysis. After purification, the recombinant S1 protein was identified by 6 serum samples of recovered SARS patients and 6 serum samples of health donors, which were collected before out-break of SARS. Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit. The recombinant S1 protein could react with all the samples from recovered SARS patients but not the control samples from healthy donors according to the results of Western blot. Conclusion:The recombinant SARS virus S1 subunit may provide a good tool for the research of immune response to SARS virus and the producing of recombinant vaccine to prevent people from SARS.
