Preliminary study on the treatment of ischemic heart disease with angiopoietin 1 gene transfection by adenovirus vector in rabbits
- VernacularTitle:血管形成素1基因转染治疗兔缺血性心肌梗死的实验研究
- Author:
Shilin CHEN
;
Hua JING
;
Jieshou LI
;
Long YI
;
Baikun MA
;
Rong FANG
- Publication Type:Journal Article
- Keywords:
Myocardial infarct;
Gene therapy
- From:
Chinese Journal of Geriatrics
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the potential effects of angiopoietin 1(Ang1) via adenovirus mediated gene transfer on ischemic heart disease in rabbits. Methods Forty-five male New Zealand white rabbits underwent high positioned double-ligation of the anterior descending left coronary artery, and were divided into three groups: Ang1 group (n=15) received direct myocardium injection of Ang1 recombinant adenovirus; DMEM group (n=15) and LacZ group (n=15) received only DMEM and LacZ recombinant adenovirus as controls respectively. The myocardial infarcted size was evaluated by N-BT macroscopic standing and the degree of angiogenesis was assessed by use of immunohistochemical analysis. The echocardiographic changes were measured on before operation and the 7 th, 14 th, and 28 th postoperative days. Results Animals in three groups had no significant difference in the percentage of infarction size of left ventricle at the 7 th, 14 th day and capillary density at the 7 th day. Animals in Ang1 group showed less infarcted size than DMEM group or LacZ group at the 28 th day and higher capillary density at the 14 th and the 28 th day. The results from echocardiographic measurements showed that animals in all three groups had no significant difference in the left ventricular systolic function before operation and at the 7 th , 14 th day, but the left ventricular systolic function in Ang1 group was better than that in DMEM group or LacZ group at the the 28 th day(P
