An experimental study on the influence of nitric oxide synthase inhibitor in a rat model of Parkinson’s disease
- VernacularTitle:一氧化氮合酶抑制剂对帕金森病模型大鼠神经损伤的影响
- Author:
Yongbo ZHAO
;
Qiaoshu WANG
;
Chunni GUO
;
Aimei MA
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
Hydroxydopamines;
Nitric-oxide synthase
- From:
Chinese Journal of Geriatrics
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of nitric oxide synthase (NOS) inhibitor in a rat model of Parkinson’s disease. Methods Hemi-Parkinsonism rat model was established by stereotaxic 6-hydroxydopamine (6-OHDA) lesions in striatum in which nitric oxide synthase (NOS) was inhibited by L-Nitro-Arginine (L-NNA) and apomorphin-induced rotational behavior was measured. The immunohistochemical staining method was used to observe the change of striatal nNOS-positive neurons and nigral tyrosine hydroxylase(TH)-positive neurons. Results L-NNA dramatically protected 6-OHDA-injected rats against indices of severe injury to the nigrostriatal dopaminergic pathway, including decreases in numbers of TH-positive nigral neurons and rotational behavior. The nNOS-positive neurons showed no changes in numbers. Conclusions These results indicate that NO might mediate, in part, 6-OHDA-induced neurotoxicity and nNOS-positive neurons might resist the 6-OHDA neurotoxicity. NOS inhibitor may play a role in the protection of 6-OHDA neurons.
