?_2-AR gene cloning from human detrusor cell and the construction of its antisense eukaryotic expression vector
- VernacularTitle:膀胱逼尿肌中肾上腺素能?_2受体基因克隆与反义真核表达载体的构建
- Author:
Gang LI
;
Changcheng SUN
;
Xiaohua ZHENG
- Publication Type:Journal Article
- Keywords:
Receptors,adrenergic,beta-2;
Eukaryotic expression vector;
Antisense gene
- From:
Chinese Journal of Urology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone human beta-2 adrenoceptor gene from human bladder smooth muscle and to construct its antisense eukaryotic expression vector. Methods The ?_2-AR full length cDNA was cloned from human detrusor cells through RT-PCR and subcloned into clone vector (pUC18).The objective gene was then cut from ClaⅠ/HindⅢ sites of pUC18 with restriction endonulcease and subcloned into pLNCX vector in trans-direction.Finally the constructed ?_2-AR gene antisense expresstion vector was identified through restriction endonuclease analysis and sequencing. Results The sequence of cloned ?_2-AR full length cDNA was certified by comparison with the database of the Genebank.The constructed antisense eukaryotic expression vector was proved to be same with designed by restriction endonuclease analysis and sequencing. Conclusions ?_2-AR full length cDNA was cloned and its antisense eukaryotic expression vector was successfully constructed.This technique establishs the foundation for the further research on drug treatment of bladder dysfunction.
