Role of leader sequence for clusterin anti-apoptotic function in LNCaP cell
- VernacularTitle:Clusterin前导序列抗前列腺癌细胞凋亡作用的研究
- Author:
Yinong NIU
;
Dianqi XIN
;
Yi DING
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Apoptosis;
Clusterin
- From:
Chinese Journal of Urology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of leader sequence in anti-apoptotic action of clusterin in LNCaP cell. Methods The wild type LNCaP cells(L), LNCaP cells transfected with the control vector(M),LNCaP cells transfected with clusterin expression vector with(A) and without(B) the leader sequence were cultured.RT-PCR was used to observe the expression of clusterin mRNA in group A,B,M and L.Cultured with TNF-?,the expression of clusterin mRNA in group L was measured,MTT and ELISA were used to determine the status of cell proliferation and apoptosis of the 4 groups. Results The expression of clusterin mRNA in group A and B was significantly higher than that in group L and M (all P 0.05).Clusterin mRNA of group L transiently elevated after treated with TNF-? for 2 h( A =15 642.0?64.3, t =-77.106, P
