Hypoxia regulates vascular endothelial growth factor and matrix metalloproteinase-2 in oral squamous cell carcinoma cell lines.
- VernacularTitle:低氧对口腔鳞癌细胞系血管内皮生长因子和MMP-2的影响
- Author:
Xiaoming JIN
;
Yaxin LI
;
Jinrong LI
- Publication Type:Journal Article
- Keywords:
Oral neoplasms;
Vascular endothelial growth factor;
Matrix metalloproteinase;
Hypoxia
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the role of hypoxia in the regulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 2 (MMP 2) in oral squamous cell carcinoma cell lines. Methods: Oral squamous cell carcinoma TSCCa and TNM cells were treated by hypoxia at ?=1% of oxygen for 4～12 h. VEGF and MMP 2 in the cells were quantitatively determined by enzymelinked immunosorbent assay (ELISA). The mRNA expression of VEGF and MMP 2 in the two cell lines was evaluated by semi quantitative RT PCR. Results: In both cell lines VEGF and MMP 2 were increased after 4 hour hypoxia treatment (P
