Separating cultivating and osteogeneric identifying of bone marrow stromal cells in rabbit
- VernacularTitle:兔骨髓基质细胞的分离培养
- Author:
Yongxian ZHANG
;
Jinfang CAI
;
Jiwen TANG
;
Xiaoyang LIU
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Cell culture;
Bone tissue engineering;
Rabbit
- From:
Chinese Journal of Microsurgery
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search a kind of ideal seeded cell of bone tissue engineering. Methods Bone marrow tissue was fetched from rabbit,got bone marrow stromal cells by the method of density centrifuge,observed the conformation,growing feature and osteogeneric capability of the cells during culture in vitro by light microscope,transmission electron microscope and measure of osteogeneric capability. Results Most of the cultivated bone marrow stromal cells were triangle-like or shuttle-like cells. They growed and breeded rapidly,and had osteogeneric capability. They were easy to differentiate to osteoblasts. Conclusion The cultivated bone marrow stromal cells are matured ones,and could become the more ideal seeded cells for bone tissue engineering.
