Study on Protective Effects of TFBP on Human Immortalized Keratinocytes Line HaCaT
- VernacularTitle:构树叶总黄酮对表皮细胞防护作用研究
- Author:
Xueying YANG
;
Rui HE
;
Yuguang CAO
- Publication Type:Journal Article
- Keywords:
Malondialdehyde;
Lead acetate;
Sodium arsenite;
Superoxide dismutase;
Glutathione peroxidase;
Total flzvonoids of broussonetia papyrifera
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the oxidative damage of lead acetate and sodium arsenite to human immortalized keratinocytes line HaCaT and the protective effects of TFBP extracted from the leaves of broussoneria papyifera. Methods Cultured immotlalized keratinocytes line HaCaT were treated by 0.1 mmol/L lead acetate and 5.0 ?mol/L sodium arsenite respectively,and 0～200 mg/L TFBP were added to the culture media at the same time. The contents of malondialdehyde(MDA?雪,the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the cultured HaCaT cells were determined. The protective effects of TFBP at different concentrations were evaluated. Results 0.1 mmol/L lead acetate caused oxidative damage to HaCaT cells markedly. When the concentrations were increased to more than 100 mg/L,TFBP had certain protective effects from the damage induced by lead acetate with the decrease of the MDA levels from 4.23 ?mol/L to 1.87 ?mol/L and the increase of the SOD levels from 25.90 U/mg Pro to 37.12 U/mg Pro,while the activities of GSH-Px showed no significant change. The activities of SOD and GSH-Px were increased in the cultured cells treated by sodium arsenite with 150 mg/L and 200 mg/L TFBP. Conclusion Lead acetate and sodium arsenite could cause significant oxidative damage to HaCaT cells and TFBP had certain protective effects on the cells from the oxidative damage induced by lead acetate and sodium arsenite under the conditions of this study.
